STRESS MARQ/STRESS XPRESS®αB晶体蛋白ELISA试剂盒/SKT-123-96/96孔
商品编号：
SKT-123-96
市场价：
¥12880.00
美元价：
7728.00
产品分类：
酚
公司分类：
hydroxybenzene
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | AlphaBCrystallinELISAKit |
| Description | ColorimetricdetectionofAlphaBCrystallin |
| SpeciesReactivity | Human |
| Platform | Microplate |
| SampleTypes | Celllysates,Serum,Tissue |
| DetectionMethod | ColorimetricAssay |
| AssayType | SandwichELISA(Enzyme-linkedImmunosorbentAssay) |
| Utility | ELISAkitusedtoquantitatealphaBcrystallinconcentrationinsamples. |
| Sensitivity | 0.009ng/ml |
| AssayRange | 0.352-22.5ng/ml |
| IncubationTime | 30minutes |
| NumberofSamples | 40samplesinduplicate |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | ||||||||||||||||||||||||||||||||||||
| ShippingTemperature | BlueIce | ||||||||||||||||||||||||||||||||||||
| ProductType | ELISAKits | ||||||||||||||||||||||||||||||||||||
| AssayOverview | 1.PrepareStandardandsamplesinStandardandSampleDiluent.2.Add100µLofStandardorsampletoappropriatewells.3.CoverplatewithPlateSealerandincubateatroomtemperature(20-25°C)for1hour.4.Washplatefourtimeswith1XWashBuffer.5.Add100µLofBiotinylatedAntibodyWorkingSolutiontoeachwell.6.CoverplatewithPlateSealerandincubateatroomtemperaturefor1hour.7.Washplatefourtimeswith1XWashBuffer.8.Add100µLofStreptavidin-HRPWorkingSolutiontoeachwell.9.CoverplatewithPlateSealerandincubateatroomtemperaturefor30minutes.10.Washplatefourtimeswith1XWashBuffer.11.Add100µLofTMBSubstratetoeachwell.12.Developtheplateinthedarkatroomtemperaturefor30minutes.13.Stopreactionbyadding100µLofStopSolutiontoeachwell.14.Measureabsorbanceonaplatereaderat450nm. | ||||||||||||||||||||||||||||||||||||
| KitOverview |
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| CiteThisProduct | AlphaBCrystallinELISAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-123) |
BIOLOGicalDescription
| AlternativeNames | AACRYAELISAKit,CRYA2ELISAKit,CRYABELISAKit,CTPP2ELISAKit,HSPB5ELISAKit,NYRen27antigenELISAKit,alphacrystallinBELISAKit |
| ResearchAreas | Cancer,HeatShock |
| ScientificBackground | Thealpha-crystallinsaremajorwater-solublelensstructuralproteinsofthevertebrateeyethatarerelatedtothesmallheatshockproteinfamily.Thealpha-crystallinspossessstructuralandfunctionalsimilaritieswithHSP25andHSP27(1).Mammalianlenscystallinsaredividedintoalpha,betaandgammafamilies.alphaandbetafamiliesarefurtherdividedintoacidicandbasicgroups(alpha-Aandalpha-Brespectively).Inthelens,alpha-crystallinprimarilyfunctionstomaintainproperrefractiveindex,howeveritcanalsofunctionasamolecularchaperonethatbindstothedenaturedproteins,keepingtheminsolutionandtherebymaintainingthetranslucencyofthelens.Whencellularstressoccurs,alpha-crystallinentersits’phosphorylatedstateandmayserveastructuralcontrolfunctionandplayaroleinproteinmaintenance(2).Inadditiontotheirinteractionwithproteins,alpha-crystallinsalsointeractwithnativemoleculessuchasmembraneproteins,Golgimatrixprotein,structuralproteins,nuclearproteinsandDNA(3,4,5,6,and7).Twootherfunctionsareanautokinaseactivityandparticipationintheintracellulararchitecture,andithasalsobeenproventhatbothalpha-AandBpreventapoptosisbyinhibitingcaspases(8).Specifically,alpha-Bcystallinisfoundinmanycellsandorgansoutsidethelens,andalphaBisoverexpressedinseveralneurologicaldisordersandincelllinesunderstressconditions(9). |
| References | 1.MerckK.B.etal.(1993)JBiolChem.268:1046-1052. 2.HorwitzJ.(1992)ProcNatlAcadSciUSA89(21):10449-10453. 3.CobbB.A.andPetrashJ.M.(2002)Biochemistry.41:483-490 4.HorwitzJ.(2003)ExpEyeRes.76:145-153. 5.BullardB.etal.(2004)JBiolChem.279:7917-7924. 6.GangalumR.K.,SchIBLerM.J.andBhatS.P.(2004)JBiolChem.279:43374.43377. 7.MaddalaR.andRaoV.P.(2005)ExpCellRes.306:203-215. 8.YaungJ.,etal.(2007)MolecularVision13:566-577. 9.HeadM.W.etal.(2000)NeuropatholApplNeurobiol.26:304-312. |
ProductImages
TypicalStandardCurvefortheAlphaBCrystallinELISAKit(Enzyme-LinkedImmunosorbentAssay)StressXpress®–SKT-123.AssayType:SandwichELISA.DetectionMethod:ColorimetricAssay.AssayRange:0.352–22.5ng/mL.
ProductCitations(1)
OtherCitations
Levelofvitreousalpha-Bcrystallinineyeswithrhegmatogenousretinaldetachment.
Baba,T.,Oshitari,T.andYamamoto,S.(2014)GraefesArchClinExpOphthalmol.253(8):1251-4.
PubMedID:25311653ReactivityHumanApplications:Vitreoussamples
品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
