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当前位置: 首页 > 产品中心 > hydroxybenzene > StressMarq/Anti-Alginate Antibody [4B10-1C5]/SMC-208D-A594/100-µg
商品详细StressMarq/Anti-Alginate Antibody [4B10-1C5]/SMC-208D-A594/100-µg
StressMarq/Anti-Alginate Antibody [4B10-1C5]/SMC-208D-A594/100-µg
StressMarq/Anti-Alginate Antibody [4B10-1C5]/SMC-208D-A594/100-µg
商品编号: SMC-208D-A594
市场价: ¥7240.00
美元价: 4344.00
产地: 美国(厂家直采)
公司:
产品分类:
公司分类: hydroxybenzene
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameAlginateAntibody
Description

MouseAnti-AlginateMonoclonalIgG1Kappa

SpeciesReactivitySpeciesIndependent
ApplicationsWB,IHC
AntibodyDilutionWB(1:1000),ICC/IF(1:100);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesMouse
ImmunogenSodiumAlginateconjugatedtoKLH
Concentration1mg/ml
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,0.09%sodiumazide
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationProteinGPurified
ClonalityMonoclonal
CloneNumber4B10-1C5
IsotypeIgG1Kappa
SpecificityBindsselectivelytoaBSA-conjugatedalginate,butnottounconjugatedBSA.
CiteThisProductStressMarqBiosciencesCat#SMC-208,RRID:AB_2699250
CertificateofAnalysis1µg/mlofSMC-208wassufficientfordetectionof150ngofalginate-conjugatedBSAbycolorimetricimmunoblotanalysisusingGoatanti-mouseIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesAlginicAcidAntibody,AlginAntibody,SodiumAlginateAntibody
ResearchAreasCellSignaling,Post-translationalModifications
ScientificBackgroundSodiumalginateisusedinbiologicalexperimentsfortheimmobilizationofcellsandencapsulationduetoitsbiocompatibilityandsimplegelationwithdivalentcationssuchasCa2+.Studiessuggestthatpreparationofalginatemicrosphereswillsustainproteindeliverywithintissuescaffolds(1),aswellasinmanyothercelltypes(2).
References1.ZhaiP.,ChenX.B.,andSchreyerD.J.(2013)Biofabrication.5(1):015009.
2.SelimogluS.M.,Ayyildiz-TamisD.,GurhanI.D.,andElibolM.(2012)JBiosciBioeng.113(2):233-238.

ProductImages

<p>Immunohistochemistry analysis using Mouse Anti-Alginate Monoclonal Antibody, Clone 4B10-1C5 (SMC-208). Tissue: Burned Skin. Species: Human. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody (SMC-208) at 1:200.</p>

ImmunohistochemistryanalysisusingMouseAnti-AlginateMonoclonalAntibody,Clone4B10-1C5(SMC-208).Tissue:BurnedSkin.Species:Human.PrimaryAntibody:MouseAnti-AlginateMonoclonalAntibody(SMC-208)at1:200.

<p>Western Blot analysis of ALL BSA-Alginate Conjugate showing detection of ~250 kDa Alginate protein using Mouse Anti-Alginate Monoclonal Antibody, Clone 4B10-1C5 (SMC-208). Lane 1: MW ladder. Lane 2: 0.625ug BSA:Alginate. Load: 0.625 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody (SMC-208) at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:100 for 1 hour at RT. Color Development: TMB solution for 2 min at RT. Predicted/Observed Size: ~250 kDa.</p>

WesternBlotanalysisofALLBSA-AlginateConjugateshowingdetectionof~250kDaAlginateproteinusingMouseAnti-AlginateMonoclonalAntibody,Clone4B10-1C5(SMC-208).Lane1:MWladder.Lane2:0.625ugBSA:Alginate.Load:0.625µg.Block:5%milk+TBSTfor1houratRT.PrimaryAntibody:MouseAnti-AlginateMonoclonalAntibody(SMC-208)at1:500for1houratRT.SecondaryAntibody:HRPGoatAnti-Mouseat1:100for1houratRT.ColorDevelopment:TMBsolutionfor2minatRT.Predicted/ObservedSize:~250kDa.

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 ATTO594
Overview:
  • Highfluorescenceyield
  • HighphotostABIlity
  • Veryhydrophilic
  • Excellentsolubilityinwater
  • Verylittleaggregation
  • Newdyewithnetchargeof-1
  • MolarMass:1137g/mol

ATTO594Datasheet

 ATTO 594 Fluorophore Excitation and Emission SpectrumOpticalProperties:

λex =601 nm

λem=627nm

εmax=1.2×105

Φf= 0.85

τfl =3.5ns

Brightness=102

Laser =594nm

Filterset =TexasRed®

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。