StressMarq/Anti-Akt2 (PKB beta) Antibody/SPC-148F-FITC/400-µl-免疫在线蚂蚁淘旗下平台

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商品详细StressMarq/Anti-Akt2 (PKB beta) Antibody/SPC-148F-FITC/400-µl

StressMarq/Anti-Akt2 (PKB beta) Antibody/SPC-148F-FITC/400-µl

商品编号： SPC-148F-FITC

品牌： StressMarq Biosciences

市场价： ¥5940.00

美元价： 3564.00

产地：美国(厂家直采)

公司：

产品分类：酚

公司分类： hydroxybenzene

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Overview:

ProductName Akt2(PKBbeta)Antibody

Description

RabbitAnti-HumanAkt2(PKBbeta)Polyclonal

SpeciesReactivity Dog,Human,Monkey,Mouse,Rat,Africanclawedfrog(Xenopuslaevis),Bovine,Chicken,GuineaPig(Caviaporcellus),Hamster,Pig,Rabbit,Sheep

Applications WB,ICC/IF,ELISA

AntibodyDilution WB(1:1000),ICC/IF(1:25);optimaldilutionsforassaysshouldbedeterminedbytheuser.

HostSpecies Rabbit

ImmunogenSpecies Human

Immunogen AfiveresiduesyntheticpeptidebasedonthehumanAkt2,coupledtoKLH

Concentration 0.25mg/ml

Conjugates

AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

PerCP

Overview:

Peridinin-Chlorophyll-ProteinComplex
Smallphycobiliprotein
Isolatedfromredalgae
Largestokesshift(195nm)
MolecularWeight:35kDa

PerCPDatasheet

PerCP Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =482nm

λ_em=677nm

ε_max=1.96x10⁶

Laser =488nm

PE/ATTO594

PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm.

Overview:

Highfluorescenceyield
HighphotostABIlity
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation

PE/ATTO594Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra

OpticalProperties:

λ_ex =535nm

λ_em =627 nm

Laser = 488to561nm

FITC(Fluorescein)

Overview:

Excellentfluorescencequantumyield
Highrateofphotobleaching
Goodsolubilityinwater
Broademissionspectrum
pHdependentspectra
Molecularformula:C₂₀H₁₂O₅
Molarmass:332.3g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =494 nm

λ_em=520 nm

ε_max=7.3×10⁴

Φ_f= 0.92

τ_fl =5.0ns

Brightness= 67.2

Laser = 488 nm

Filterset = FITC

ATTO700

Overview:

Highfluorescenceyield
Excellentthermalandphotostability
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:575g/mol

ATTO700Datasheet

ATTO 700 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =700 nm

λ_em=719nm

ε_max=1.25×10⁵

Φ_f= 0.25

τ_fl =1.6ns

Brightness= 31.3

Laser =676nm

Filterset =Cy®5.5

ATTO680

Overview:

Highfluorescenceyield
Excellentthermalandphotostability
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:631g/mol

ATTO680Datasheet

ATTO 680 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =680nm

λ_em=700 nm

ε_max=1.25×10⁵

Φ_f= 0.30

τ_fl =1.7ns

Brightness=37.5

Laser =633 –676nm

Filterset =Cy®5.5

ATTO655

Overview:

Highfluorescenceyield
Highthermalandphotostability
Excellentozoneresistance
Quenchedbyelectrondonors
Veryhydrophilic
Goodsolubilityinpolarsolvents
Zwitterionicdye
MolarMass:634g/mol

ATTO655Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =663nm

λ_em=684nm

ε_max=1.25×10⁵

Φ_f= 0.30

τ_fl =1.8ns

Brightness= 37.5

Laser =633 –647nm

Filterset =Cy®5

ATTO633

Overview:

Highfluorescenceyield
Highthermalandphotostability
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
StableatpH4–11
Cationicdye,perchloratesalt
MolarMass:652.2g/mol

ATTO633Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =629nm

λ_em=657nm

ε_max=1.3×10⁵

Φ_f= 0.64

τ_fl =3.2ns

Brightness=83.2

Laser =633 nm

Filterset =Cy®5

ATTO594

Overview:

Highfluorescenceyield
Highphotostability
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation
Newdyewithnetchargeof-1
MolarMass:1137g/mol

ATTO594Datasheet

ATTO 594 Fluorophore Excitation and Emission Spectrum

OpticalProperties:

λ_ex =601 nm

λ_em=627nm

ε_max=1.2×10⁵

Φ_f= 0.85

τ_fl =3.5ns

Brightness=102

Laser =594nm

Filterset =TexasRed®

ATTO565

Overview:

Highfluorescenceyield
Highthermalandphotostability
Goodsolubilityinpolarsolvents
Excellentsolubilityinwater
Verylittleaggregation
Rhodaminedyederivative
MolarMass:611g/mol

ATTO565Datasheet

ATTO 565 Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =563nm

λ_em=592nm

ε_max=1.2×10⁵

Φ_f= 0.9

τ_fl =3.4n

Brightness=10

Laser =532nm

Filterset = TRITC

ATTO488

Overview:

Highfluorescenceyield
Highphotostability
Veryhydrophilic
Excellentsolubilityinwater
Verylittleaggregation
Newdyewithnetchargeof-1
MolarMass:804g/mol

ATTO488Datasheet

ATTO 488 Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =501nm

λ_em=523nm

ε_max=9.0×10⁴

Φ_f= 0.80

τ_fl =4.1ns

Brightness= 72

Laser = 488 nm

Filterset = FITC

ATTO390

Overview:

Highfluorescenceyield
LargeStokes-shift(89nm)
Goodphotostability
Moderatelyhydrophilic
Goodsolubilityinpolarsolvents
Coumarinderivate,uncharged
Lowmolarmass:343.42g/mol

ATTO390Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission Spectra

OpticalProperties:

λ_ex =390nm

λ_em=479nm

ε_max=2.4×10⁴

Φ_f= 0.90

τ_fl =5.0ns

Brightness= 21.6

Laser =365or405nm

APC(Allophycocyanin)

Overview:

Highquantumyield
Largephycobiliprotein
6chromophorespermolecule
Isolatedfromredalgae
MolecularWeight:105kDa

APCDatasheet

APC Fluorophore Absorption and Emission Spectrum

OpticalProperties:

λ_ex =650nm

λ_em=660nm

ε_max=7.0×10⁵

Φ_f= 0.68

Brightness= 476

Laser =594or633 nm

Filterset =Cy®5

Streptavidin

Properties:

Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinity
Molecularweight:53kDa
Formula:C₁₀H₁₆N₂O₃S
Applications:Westernblot,immunohistochemistry,andELISA

StreptavidinDatasheet

Biotin

Properties:

BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinity
Molarmass:244.31g/mol
Formula:C₁₀H₁₆N₂O₃S
Applications:Westernblot,immunohistochemistry,andELISA

BiotinDatasheet

HRP(HorserADIshperoxidase)

Properties:

Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
Readilycombineswithhydrogenperoxide(H₂O₂)toformHRP-H₂O₂complexwhichcanoxidizevarioushydrogendonors
Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
- Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
- Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
Highturnoverrateenablesrapidgenerationofastrongsignal
44kDaglycoprotein
Extinctioncoefficient:100(403nm)
Applications:Westernblot,immunohistochemistry,andELISA

HRPDatasheet

AP(AlkalinePhosphatase)

Properties:

Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenols
Commonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligation
Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproducts
- Fluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproducts
Molecularweight:140kDa
Applications:Westernblot,immunohistochemistry,andELISA

APDatasheet

R-PE(R-Phycoerythrin)

Overview:

Broadexcitationspectrum
Highquantumyield
Photostable
Memberofthephycobiliproteinfamily
Isolatedfromredalgae
Excellentsolubilityinwater
MolecularWeight:250kDa

R-PEDatasheet

R-PE Fluorophore Excitation and Emission Spectra

OpticalProperties:

λ_ex =565nm

λ_em=575nm

ε_max=2.0×10⁶

Φ_f= 0.84

Brightness=1.68x10³

Laser = 488to561nm

Filterset = TRITC

Properties

StorageBuffer	TBS,50%glycerol,0.09%sodiumazide
StorageTemperature	-20ºC
ShippingTemperature	BlueIceor4ºC
Purification	ProteinApurified
Clonality	Polyclonal
Specificity	Detects~65kDa.DoesnotreactwithPKBα(Akt1)orPKBγ(Akt3).
CiteThisProduct	StressMarqBiosciencesCat#SPC-148,RRID:AB_2225163
CertificateofAnalysis	A1:3000dilutionofSPC-148wassufficientfordetectionofPKBβin20µgofHeLacelllysatebyECLimmunoblotanalysis.

BIOLOGicalDescription

AlternativeNames	AKT2Antibody,PKBAntibody,PKBbetaAntibody,PRKBBAntibody,proteinkinaseAkt2Antibody,RACBETAAntibody
ResearchAreas	CellSignaling,Phosphorylation,Post-translationalModifications
CellularLocalization	Cytoplasm,Cellmembrane,Nucleus
AccessionNumber	NP_001617.1
GeneID	208
SwissProt	P31751
ScientificBackground	ProteinkinaseBorAkt(PKB/Akt)isaserine/threoninekinase,whichinmammalscomprisesthreehighlyhomologousmembersknownasPKBalpha(Akt1),PKBbeta(Akt2)andPKBgamma(Akt3).PKB/Aktisagrowth-factor-regulatedproteinkinasewhichcontainsaPleckstrinHomology(PH)domainwhichmightbeimportantfordimerizationofthekinase.Bindingofphosphoinositide3-OHkinaseproductstothepleckstrinhomologydomainresultsintranslocationofPKB/Akttotheplasmamembranewhereitisactivatedbyphosphorylationbyupstreamkinasesincludingthephosphoinoside-dependentkinase1(PDK1)andPDK2(1-3).MyelinbasicproteinandhistoneH2BareinvitrosubstratesforPKB,whileglycogensynthasekinase-3hasbeenimplicatedasaphysiologicaltarget(4).Keyrolesforthisenzymecanbefoundincellularprocessessuchasglucosemetabolism,cellproliferation,apoptosis,transcriptionandcellmigration(1-3).
References	1.DownwardJ.(1998).CurrOpinCellBiol.10(2):262-7. 2.BrazilD.P.,andHemmingsB.A.(2001).BiochemSci.26(11):657-64. 3.NicholsonK.M.,AndersonN.G.(2002).CellSignal.14(5):381-95. 4.Hardie,G.,Hanks,S.(1995).TheProteinKinaseFactsBook,Protein-SerineKinasesAcademicPressLimited,SanDiego,CA418pps.

ProductImages

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148) at 1:25 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-AKT2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.</p>

Immunocytochemistry/ImmunofluorescenceanalysisusingRabbitAnti-AKT2PolyclonalAntibody(SPC-148).Tissue:HeatShockedHeLaCells.Species:Human.Fixation:2%Formaldehydefor20minatRT.PrimaryAntibody:RabbitAnti-AKT2PolyclonalAntibody(SPC-148)at1:25for12hoursat4°C.SecondaryAntibody:FITCGoatAnti-Rabbit(green)at1:200for2hoursatRT.Counterstain:DAPI(blue)nuclearstainat1:40000for2hoursatRT.Localization:Cytoplasm.Nucleus.Magnification:100x.(A)DAPI(blue)nuclearstain.(B)Anti-AKT2Antibody.(C)Composite.HeatShockedat42°Cfor1h.

<p>Western blot analysis of multiple Cell line lysates showing detection of AKT2 protein using Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.</p>

WesternblotanalysisofmultipleCelllinelysatesshowingdetectionofAKT2proteinusingRabbitAnti-AKT2PolyclonalAntibody(SPC-148).Load:15µgprotein.Block:1.5%BSAfor30minutesatRT.PrimaryAntibody:RabbitAnti-AKT2PolyclonalAntibody(SPC-148)at1:1000for2hoursatRT.SecondaryAntibody:DonkeyAnti-RabbitIgG:HRPfor1houratRT.

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FITC(Fluorescein)

Overview:

Excellentfluorescencequantumyield
Highrateofphotobleaching
Goodsolubilityinwater
Broademissionspectrum
pHdependentspectra
Molecularformula:C₂₀H₁₂O₅
Molarmass:332.3g/mol

FITC-Fluorescent-conjugate

OpticalProperties:

λ_ex =494 nm

λ_em=520 nm

ε_max=7.3×10⁴

Φ_f= 0.92

τ_fl =5.0ns

Brightness= 67.2

Laser = 488 nm

Filterset = FITC

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