4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > hydroxybenzene > StressMarq/Anti-Akt2 (PKB beta) Antibody/SPC-148F-ALP/400-µl
商品详细StressMarq/Anti-Akt2 (PKB beta) Antibody/SPC-148F-ALP/400-µl
StressMarq/Anti-Akt2 (PKB beta) Antibody/SPC-148F-ALP/400-µl
StressMarq/Anti-Akt2 (PKB beta) Antibody/SPC-148F-ALP/400-µl
商品编号: SPC-148F-ALP
市场价: ¥5980.00
美元价: 3588.00
产地: 美国(厂家直采)
公司:
产品分类:
公司分类: hydroxybenzene
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name Akt2 (PKB beta) Antibody
Description

Rabbit Anti-Human Akt2 (PKB beta) Polyclonal

Species Reactivity Dog, Human, Monkey, Mouse, Rat, African clawed frog (Xenopus laevis), Bovine, Chicken, Guinea Pig (Cavia porcellus), Hamster, Pig, Rabbit, Sheep
Applications WB, ICC/IF, ELISA
Antibody Dilution WB (1:1000), ICC/IF (1:25); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen A five residue synthetic peptide based on the human Akt2, coupled to KLH
Concentration 0.25 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer TBS, 50% glycerol, 0.09% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein A purified
Clonality Polyclonal
Specificity Detects ~65kDa. Does not react with PKBα (Akt1) or PKBγ (Akt3).
Cite This Product StressMarq Biosciences Cat# SPC-148, RRID: AB_2225163
Certificate of Analysis A 1:3000 dilution of SPC-148 was sufficient for detection of PKBβ in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Biological Description

Alternative Names AKT2 Antibody, PKB Antibody, PKB beta Antibody, PRKBB Antibody, protein kinase Akt2 Antibody, RAC BETA Antibody
Research Areas Cell Signaling, Phosphorylation, Post-translational Modifications
Cellular Localization Cytoplasm, Cell membrane, Nucleus
Accession Number NP_001617.1
Gene ID 208
Swiss Prot P31751
Scientific Background Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).
References 1. Downward J. (1998). Curr Opin Cell Biol. 10(2): 262-7.
2. Brazil D.P., and Hemmings B.A. (2001). Biochem Sci. 26(11):657-64.
3. Nicholson K.M., Anderson N.G. (2002). Cell Signal. 14(5): 381-95.
4. Hardie, G., Hanks, S. (1995). The Protein Kinase Facts Book, Protein-Serine Kinases Academic Press Limited, San Diego, CA 418pps.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148) at 1:25 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-AKT2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.</p>

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148) at 1:25 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-AKT2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

<p>Western blot analysis of multiple Cell line lysates showing detection of AKT2 protein using Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.</p>

Western blot analysis of multiple Cell line lysates showing detection of AKT2 protein using Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Product Citations (0)

Currently there are no citations for this product.

AP (Alkaline Phosphatase)

Properties:

  • Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
  • Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
    • Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
  • Molecular weight: 140 kDa
  • Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。