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当前位置: 首页 > 产品中心 > hydroxybenzene > StressMarq/Anti-AKT1 Antibody (pTyr315)/SPC-911D-P594/100-µl
商品详细StressMarq/Anti-AKT1 Antibody (pTyr315)/SPC-911D-P594/100-µl
StressMarq/Anti-AKT1 Antibody (pTyr315)/SPC-911D-P594/100-µl
StressMarq/Anti-AKT1 Antibody (pTyr315)/SPC-911D-P594/100-µl
商品编号: SPC-911D-P594
市场价: ¥6980.00
美元价: 4188.00
产地: 美国(厂家直采)
公司:
产品分类:
公司分类: hydroxybenzene
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name AKT1 Antibody (pTyr315)
Description

Rabbit Anti-Human AKT1 (pTyr315) Polyclonal

Species Reactivity Human
Applications WB, AM
Antibody Dilution WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen A phospho-specific peptide corresponding to residues surrounding Tyr315 of human Akt1 (AA312-318)
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Peptide Affinity Purified
Clonality Polyclonal
Specificity Detects 55.686 kDa.
Cite This Product StressMarq Biosciences Cat# SPC-911, RRID: AB_2706797
Certificate of Analysis A 1:250 dilution of SPC-911 was sufficient for detection of AKT1 (pTyr315) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names AKT1 Antibody, AKT1_Human Antibody, PKB Antibody, Protein Kinase B Alpha Antibody, RAC Antibody
Research Areas Cancer, Apoptosis, Cell Signaling, Metabolism, Metabolism processes, PKB / AKT, Protein Phosphorylation, Serine / Threonine Kinases
Cellular Localization Cytoplasm, Cell membrane, Nucleus
Accession Number NP_005154
Gene ID 207
Swiss Prot P31749
Scientific Background AKT1 is a protein-serine/threonine kinase which is inactive in the cytoplasm. When PI3K is activated by extracellular growth factors, AKT1 is recruited to the cell membrane and phosphorylated to become active. This leads to the induction of survival, proliferation, angiogenesis and metabolism pathways. AKT1 is also involved in cell-cycle progression via its inhibitory phosphorylation of cyclin-dependent kinases and activatory phosphorylation of mTOR. Upregulation of the PI3K/AKT pathways has been implicated in a variety of cancers.

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 PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation

PE/ATTO 594 Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission SpectraOptical Properties:

λex = 535 nm

λem = 627 nm

Laser = 488 to 561 nm

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。