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当前位置: 首页 > 产品中心 > hydroxybenzene > StressMarq/Anti-AKT1 Antibody (pTyr315)/SPC-911D-HRP/100-µl
商品详细StressMarq/Anti-AKT1 Antibody (pTyr315)/SPC-911D-HRP/100-µl
StressMarq/Anti-AKT1 Antibody (pTyr315)/SPC-911D-HRP/100-µl
StressMarq/Anti-AKT1 Antibody (pTyr315)/SPC-911D-HRP/100-µl
商品编号: SPC-911D-HRP
市场价: ¥6600.00
美元价: 3960.00
产地: 美国(厂家直采)
公司:
产品分类:
公司分类: hydroxybenzene
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductNameAKT1Antibody(pTyr315)
Description

RabbitAnti-HumanAKT1(pTyr315)Polyclonal

SpeciesReactivityHuman
ApplicationsWB,AM
AntibodyDilutionWB(1:250);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenAphospho-specificpeptidecorrespondingtoresiduessurroundingTyr315ofhumanAkt1(AA312-318)
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferPBSpH7.4,50%glycerol,0.025%Thimerosal
StorageTemperature-20ºC
ShippingTemperatureBlueIceor4ºC
PurificationPeptideAffinityPurified
ClonalityPolyclonal
SpecificityDetects55.686kDa.
CiteThisProductStressMarqBiosciencesCat#SPC-911,RRID:AB_2706797
CertificateofAnalysisA1:250dilutionofSPC-911wassufficientfordetectionofAKT1(pTyr315)in10µgofHeLacelllysatebyECLimmunoblotanalysisusinggoatanti-rabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNamesAKT1Antibody,AKT1_HumanAntibody,PKBAntibody,ProteinKinaseBAlphaAntibody,RACAntibody
ResearchAreasCancer,Apoptosis,CellSignaling,Metabolism,Metabolismprocesses,PKB/AKT,ProteinPhosphorylation,Serine/ThreonineKinases
CellularLocalizationCytoplasm,Cellmembrane,Nucleus
AccessionNumberNP_005154
GeneID207
SwissProtP31749
ScientificBackgroundAKT1isaprotein-serine/threoninekinasewhichisinactiveinthecytoplasm.WhenPI3Kisactivatedbyextracellulargrowthfactors,AKT1isrecruitedtothecellmembraneandphosphorylatedtobecomeactive.Thisleadstotheinductionofsurvival,proliferation,angiogenesisandmetabolismpathways.AKT1isalsoinvolvedincell-cycleprogressionviaitsinhibitoryphosphorylationofcyclin-dependentkinasesandactivatoryphosphorylationofmTOR.UpregulationofthePI3K/AKTpathwayshasbeenimplicatedinavarietyofcancers.

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HRP(HorserADIshperoxidase)

Properties:

  • Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
  • Readilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonors
  • Catalyzestheconversionof:
    • Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
    • Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
    • Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
  • Highturnoverrateenablesrapidgenerationofastrongsignal
  • 44kDaglycoprotein
  • Extinctioncoefficient:100(403nm)
  • Applications:Westernblot,immunohistochemistry,andELISA

HRPDatasheet

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。