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当前位置: 首页 > 产品中心 > hydroxybenzene > StressMarq/Anti-AKT1 Antibody/SPC-1100D-HRP/100-µl
商品详细StressMarq/Anti-AKT1 Antibody/SPC-1100D-HRP/100-µl
StressMarq/Anti-AKT1 Antibody/SPC-1100D-HRP/100-µl
StressMarq/Anti-AKT1 Antibody/SPC-1100D-HRP/100-µl
商品编号: SPC-1100D-HRP
市场价: ¥6600.00
美元价: 3960.00
产地: 美国(厂家直采)
公司:
产品分类:
公司分类: hydroxybenzene
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name AKT1 Antibody
Description

Rabbit Anti-Human AKT1 Polyclonal

Species Reactivity Human, Mouse
Applications WB, AM
Antibody Dilution WB (1:250); optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Human
Immunogen Synthetic peptide of human Akt1 (AA133-147)
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Peptide Affinity Purified
Clonality Polyclonal
Specificity Detects 55.716 kDa.
Cite This Product StressMarq Biosciences Cat# SPC-1100, RRID: AB_2710199
Certificate of Analysis A 1:250 dilution of SPC-1100 was sufficient for detection of AKT1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Biological Description

Alternative Names AKT1 Antibody, AKT1_Human Antibody, PKB Antibody, Protein Kinase B Alpha Antibody, RAC Antibody
Cellular Localization Cytoplasm, Cell membrane, Nucleus
Accession Number NP_005154
Gene ID 207
Swiss Prot P31749
Scientific Background AKT1 is a protein-serine/threonine kinase which is inactive in the cytoplasm. When PI3K is activated by extracellular growth factors, AKT1 is recruited to the cell membrane and phosphorylated to become active. This leads to the induction of survival, proliferation, angiogenesis and metabolism pathways. AKT1 is also involved in cell-cycle progression via its inhibitory phosphorylation of cyclin-dependent kinases and activatory phosphorylation of mTOR. Upregulation of the PI3K/AKT pathways has been implicated in a variety of cancers.

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HRP (Horseradish peroxidase)

Properties:

  • Enzymatic activity is used to amplify weak signals and increase visibility of a target
  • Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
    • Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
    • Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
  • High turnover rate enables rapid generation of a strong signal
  • 44 kDa glycoprotein
  • Extinction coefficient: 100 (403 nm)
  • Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。