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当前位置: 首页 > 产品中心 > Electrophoretic_buffer > StressMarq/MOLECULAR SIGNATURE® Anti-7-Ketocholesterol Antibody [3F7]/SMC-513D-RPE/100-µg
商品详细StressMarq/MOLECULAR SIGNATURE® Anti-7-Ketocholesterol Antibody [3F7]/SMC-513D-RPE/100-µg
StressMarq/MOLECULAR SIGNATURE® Anti-7-Ketocholesterol Antibody [3F7]/SMC-513D-RPE/100-µg
StressMarq/MOLECULAR SIGNATURE® Anti-7-Ketocholesterol Antibody [3F7]/SMC-513D-RPE/100-µg
商品编号: SMC-513D-RPE
市场价: ¥8180.00
美元价: 4908.00
产地: 美国(厂家直采)
公司:
产品分类: 电泳缓冲液
公司分类: Electrophoretic_buffer
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

Product Name 7-Ketocholesterol Antibody
Description

Mouse Anti-7-Ketocholesterol (7-KC) Monoclonal IgG3

Species Reactivity Species Independent
Applications WB, ICC/IF, ELISA
Antibody Dilution WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Kemocyanin (KLH).
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

Properties

Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number 3F7
Isotype IgG3
Specificity Specific for 7-ketocholesterol modified proteins.
Cite This Product StressMarq Biosciences Cat# SMC-513, RRID: AB_2702873
Certificate of Analysis A 1:1000 dilution of SMC-513 was sufficient for detection of 7-Ketocholesterol in 2 µg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Biological Description

Alternative Names 7-Ketocholesterol [7-KC] Antibody, 7-KC Antibody, 7-ketocholesterol (7-KC) Antibody, 7KC Antibody, 7KC-Modified Keyhole-Lympet Hemocyanine Antibody, 7KC-Modified KLH Antibody
Research Areas Cancer, Lipid peroxidation, Oxidative Stress

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7 (SMC-513). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody (SMC-513) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) – Untreated. (B,D,F,H) – Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7 (SMC-513). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody (SMC-513) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) – Untreated. (B,D,F,H) – Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

<p>Western Blot analysis of 7-Ketocholesterol-BSA Conjugate showing detection of 67 kDa 7-Ketocholesterol-BSA using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7 (SMC-513). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (2.0 µg). Lane 4: 7-ketocholesterol-BSA (0.5 µg). Lane 5: 7-ketocholesterol-BSA (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody (SMC-513) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.</p>

Western Blot analysis of 7-Ketocholesterol-BSA Conjugate showing detection of 67 kDa 7-Ketocholesterol-BSA using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7 (SMC-513). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (2.0 µg). Lane 4: 7-ketocholesterol-BSA (0.5 µg). Lane 5: 7-ketocholesterol-BSA (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody (SMC-513) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

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  R-PE (R-Phycoerythrin)
Overview:

  • Broad excitation spectrum
  • High quantum yield
  • Photostable
  • Member of the phycobiliprotein family
  • Isolated from red algae
  • Excellent solubility in water
  • Molecular Weight: 250 kDa

R-PE Datasheet

 R-PE Fluorophore Excitation and Emission SpectraOptical Properties:

λex = 565 nm

λem = 575 nm

εmax = 2.0×106

Φf = 0.84

Brightness = 1.68 x 103

Laser = 488 to 561 nm

Filter set = TRITC

 

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。