| AntibodyDilution | WB(1:1000);ICC/IF(1:50);ELISA(1:1000);optimaldilutionsforassaysshouldbedeterminedbytheuser. |
| Conjugates | AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated
| PerCP | Overview:- Peridinin-Chlorophyll-ProteinComplex
- Smallphycobiliprotein
- Isolatedfromredalgae
- Largestokesshift(195nm)
- MolecularWeight:35kDa
PerCPDatasheet |  | OpticalProperties: λex =482nm λem=677nm εmax=1.96x106 Laser =488nm | PE/ATTO594 | | PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm. | Overview:- Highfluorescenceyield
- HighphotostABIlity
- Veryhydrophilic
- Excellentsolubilityinwater
- Verylittleaggregation
PE/ATTO594Datasheet |  | OpticalProperties: λex =535nm λem =627 nm Laser = 488to561nm | FITC(Fluorescein) | Overview:- Excellentfluorescencequantumyield
- Highrateofphotobleaching
- Goodsolubilityinwater
- Broademissionspectrum
- pHdependentspectra
- Molecularformula:C20H12O5
- Molarmass:332.3g/mol
FITC-Fluorescent-conjugate |  | OpticalProperties: λex =494 nm λem=520 nm εmax=7.3×104 Φf= 0.92 τfl =5.0ns Brightness= 67.2 Laser = 488 nm Filterset = FITC | ATTO700 | Overview:- Highfluorescenceyield
- Excellentthermalandphotostability
- Quenchedbyelectrondonors
- Veryhydrophilic
- Goodsolubilityinpolarsolvents
- Zwitterionicdye
- MolarMass:575g/mol
ATTO700Datasheet |  | OpticalProperties: λex =700 nm λem=719nm εmax=1.25×105 Φf= 0.25 τfl =1.6ns Brightness= 31.3 Laser =676nm Filterset =Cy®5.5 | ATTO680 | Overview:- Highfluorescenceyield
- Excellentthermalandphotostability
- Quenchedbyelectrondonors
- Veryhydrophilic
- Goodsolubilityinpolarsolvents
- Zwitterionicdye
- MolarMass:631g/mol
ATTO680Datasheet |  | OpticalProperties: λex =680nm λem=700 nm εmax=1.25×105 Φf= 0.30 τfl =1.7ns Brightness=37.5 Laser =633 –676nm Filterset =Cy®5.5 | ATTO655 | Overview:- Highfluorescenceyield
- Highthermalandphotostability
- Excellentozoneresistance
- Quenchedbyelectrondonors
- Veryhydrophilic
- Goodsolubilityinpolarsolvents
- Zwitterionicdye
- MolarMass:634g/mol
ATTO655Datasheet |  | OpticalProperties: λex =663nm λem=684nm εmax=1.25×105 Φf= 0.30 τfl =1.8ns Brightness= 37.5 Laser =633 –647nm Filterset =Cy®5 | ATTO633 | Overview:- Highfluorescenceyield
- Highthermalandphotostability
- Moderatelyhydrophilic
- Goodsolubilityinpolarsolvents
- StableatpH4–11
- Cationicdye,perchloratesalt
- MolarMass:652.2g/mol
ATTO633Datasheet |  | OpticalProperties: λex =629nm λem=657nm εmax=1.3×105 Φf= 0.64 τfl =3.2ns Brightness=83.2 Laser =633 nm Filterset =Cy®5 | ATTO594 | Overview:- Highfluorescenceyield
- Highphotostability
- Veryhydrophilic
- Excellentsolubilityinwater
- Verylittleaggregation
- Newdyewithnetchargeof-1
- MolarMass:1137g/mol
ATTO594Datasheet |  | OpticalProperties: λex =601 nm λem=627nm εmax=1.2×105 Φf= 0.85 τfl =3.5ns Brightness=102 Laser =594nm Filterset =TexasRed® | ATTO565 | Overview:- Highfluorescenceyield
- Highthermalandphotostability
- Goodsolubilityinpolarsolvents
- Excellentsolubilityinwater
- Verylittleaggregation
- Rhodaminedyederivative
- MolarMass:611g/mol
ATTO565Datasheet |  | OpticalProperties: λex =563nm λem=592nm εmax=1.2×105 Φf= 0.9 τfl =3.4n Brightness=10 Laser =532nm Filterset = TRITC | ATTO488 | Overview:- Highfluorescenceyield
- Highphotostability
- Veryhydrophilic
- Excellentsolubilityinwater
- Verylittleaggregation
- Newdyewithnetchargeof-1
- MolarMass:804g/mol
ATTO488Datasheet |  | OpticalProperties: λex =501nm λem=523nm εmax=9.0×104 Φf= 0.80 τfl =4.1ns Brightness= 72 Laser = 488 nm Filterset = FITC | ATTO390 | Overview:- Highfluorescenceyield
- LargeStokes-shift(89nm)
- Goodphotostability
- Moderatelyhydrophilic
- Goodsolubilityinpolarsolvents
- Coumarinderivate,uncharged
- Lowmolarmass:343.42g/mol
ATTO390Datasheet |  | OpticalProperties: λex =390nm λem=479nm εmax=2.4×104 Φf= 0.90 τfl =5.0ns Brightness= 21.6 Laser =365or405nm | APC(Allophycocyanin) | Overview:- Highquantumyield
- Largephycobiliprotein
- 6chromophorespermolecule
- Isolatedfromredalgae
- MolecularWeight:105kDa
APCDatasheet |  | OpticalProperties: λex =650nm λem=660nm εmax=7.0×105 Φf= 0.68 Brightness= 476 Laser =594or633 nm Filterset =Cy®5 StreptavidinProperties: - Homo-tetramericproteinpurifiedfromStreptomycesavidiniiwhichbindsfourbiotinmoleculeswithextremelyhighaffinity
- Molecularweight:53kDa
- Formula:C10H16N2O3S
- Applications:Westernblot,immunohistochemistry,andELISA
StreptavidinDatasheet Biotin Properties: - BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinity
- Molarmass:244.31g/mol
- Formula:C10H16N2O3S
- Applications:Westernblot,immunohistochemistry,andELISA
BiotinDatasheet HRP(HorserADIshperoxidase)Properties: - Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
- Readilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonors
- Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
- Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
- Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
- Highturnoverrateenablesrapidgenerationofastrongsignal
- 44kDaglycoprotein
- Extinctioncoefficient:100(403nm)
- Applications:Westernblot,immunohistochemistry,andELISA
HRPDatasheet AP(AlkalinePhosphatase)Properties: - Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenols
- Commonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligation
- Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproducts
- Fluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproducts
- Molecularweight:140kDa
- Applications:Westernblot,immunohistochemistry,andELISA
APDatasheet | R-PE(R-Phycoerythrin) | Overview:- Broadexcitationspectrum
- Highquantumyield
- Photostable
- Memberofthephycobiliproteinfamily
- Isolatedfromredalgae
- Excellentsolubilityinwater
- MolecularWeight:250kDa
R-PEDatasheet |  | OpticalProperties: λex =565nm λem=575nm εmax=2.0×106 Φf= 0.84 Brightness=1.68x103 Laser = 488to561nm Filterset = TRITC ProductImages Immunocytochemistry/ImmunofluorescenceanalysisusingMouseAnti-7-KetocholesterolMonoclonalAntibody,Clone3F7(SMC-513).Tissue:Embryonickidneycells(HEK293).Species:Human.Fixation:5%Formaldehydefor5min.PrimaryAntibody:MouseAnti-7-KetocholesterolMonoclonalAntibody(SMC-513)at1:50for30-60minatRT.SecondaryAntibody:GoatAnti-MouseAlexaFluor488at1:1500for30-60minatRT.Counterstain:PhalloidinAlexaFluor633F-Actinstain;DAPI(blue)nuclearstainat1:250,1:50000for30-60minatRT.Magnification:20X(2XZoom).(A,C,E,G)–Untreated.(B,D,F,H)–Cellsculturedovernightwith50µMH2O2.(A,B)DAPI(blue)nuclearstain.(C,D)PhalloidinAlexFluor633F-Actinstain.(E,F)7-KetocholesterolAntibody.(G,H)Composite.Courtesyof:Dr.RobertBurke,UniversityofVictoria.  WesternBlotanalysisof7-Ketocholesterol-BSAConjugateshowingdetectionof67kDa7-Ketocholesterol-BSAusingMouseAnti-7-KetocholesterolMonoclonalAntibody,Clone3F7(SMC-513).Lane1:MolecularWeightLadder(MW).Lane2:BSA(0.5µg).Lane3:BSA(2.0µg).Lane4:7-ketocholesterol-BSA(0.5µg).Lane5:7-ketocholesterol-BSA(2.0µg).Block:5%SkimMilkinTBST.PrimaryAntibody:MouseAnti-7-KetocholesterolMonoclonalAntibody(SMC-513)at1:1000for2hoursatRT.SecondaryAntibody:GoatAnti-MouseIgG:HRPat1:2000for60minatRT.ColorDevelopment:ECLsolutionfor5mininRT.Predicted/ObservedSize:67kDa. ProductCitations(0)Currentlytherearenocitationsforthisproduct. HRP(Horseradishperoxidase)Properties: - Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
- Readilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonors
- Catalyzestheconversionof:
- Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
- Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
- Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
- Highturnoverrateenablesrapidgenerationofastrongsignal
- 44kDaglycoprotein
- Extinctioncoefficient:100(403nm)
- Applications:Westernblot,immunohistochemistry,andELISA
HRPDatasheet
品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
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![StressMarq/MOLECULAR SIGNATURE® Anti-7-Ketocholesterol Antibody [3F7]/SMC-513D-HRP/100-µg](https://www.ebiomall.cn/images/StressMarq/201710/SMC-513_7-Ketocholesterol_Antibody_3F7_ICC-IF_Human_Embryonic-kidney-cells-HEK293_Composite_1-150x150.png)
