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当前位置: 首页 > 产品中心 > Electrophoretic_buffer > StressMarq/Anti-20S Proteasome Antibody/SPC-215D-HRP/100-µl
商品详细StressMarq/Anti-20S Proteasome Antibody/SPC-215D-HRP/100-µl
StressMarq/Anti-20S Proteasome Antibody/SPC-215D-HRP/100-µl
StressMarq/Anti-20S Proteasome Antibody/SPC-215D-HRP/100-µl
商品编号: SPC-215D-HRP
市场价: ¥6400.00
美元价: 3840.00
产地: 美国(厂家直采)
公司:
产品分类: 电泳缓冲液
公司分类: Electrophoretic_buffer
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Overview:

ProductName20SProteasomeAntibody
Description

RabbitAnti-Human20SProteasomePolyclonal

SpeciesReactivityHuman,Mouse
ApplicationsWB,ICC/IF,IP
AntibodyDilutionWB(1:5000);optimaldilutionsforassaysshouldbedeterminedbytheuser.
HostSpeciesRabbit
ImmunogenSpeciesHuman
ImmunogenHuman20Sproteasomeextractedfromerythrocytes
ConjugatesAlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated

Properties

StorageBufferRabbitAntiserum,0.1%sodiumazide
StorageTemperature4ºC
ShippingTemperatureBlueIceor4ºC
PurificationRabbitantiserum
ClonalityPolyclonal
SpecificityDetectsmultiple20Sproteasomesubunits.
CiteThisProductStressMarqBiosciencesCat#SPC-215,RRID:AB_2704379
CertificateofAnalysisA1:1000dilutionofSPC-215wassufficientfordetectionof20Sproteasome‘core’subunitsin0.1µgofhuman26SproteasomebyelectRochemiluminescenceanalysisusinggoatanti-rabbitIgG:HRPasthesecondaryantibody.

BIOLOGicalDescription

AlternativeNames20ScoreparticleAntibody,MacropainAntibody,MulticatalyticendopeptidasecomplexsubunitAntibody,ProsomesubunitAntibody,Proteasomesubunitalphatype1Antibody,Proteasomesubunitalphatype5Antibody,Proteasomesubunitalphatype7Antibody,ProteasomesubunitAntibody,Proteasomesubunitbetatype1Antibody,Proteasomesubunitbetatype5Antibody,Proteasomesubunitbetatype6Antibody,Proteasomesubunitbetatype7Antibody,PSMA1Antibody,PSMA5Antibody,PSMA7Antibody,PSMB1Antibody,PSMB5Antibody,PSMB6Antibody,PSMB7Antibody,ZETAAntibody
ResearchAreasCancer,Apoptosis
CellularLocalizationCytoplasm
ScientificBackgroundProteasomesfunctioninthedegradationofunusableordamagedcellularproteins.Proteasomecomplexesarethereforelocalizedinthenucleusandcytoplasmofeukaryoticcells.Themostcommonlyfoundproteasomeinmammaliancytosolisthe26Sproteasome,whichismadeupofone20Sproteasomecoresubunitandtwo19Ssubunits.The20Scoreparticleisinitselfmadeupoftwoalphasubunitsandtwobetasubunits,whichrepresentthecatalyticcomponent.Proteinsdestinedfordegradationarefirstlabeledbypolyubiquitinationbeforetheyentertheproteasomecoreandarebrokendown.
References1.SaekiY.,&TanakaK.(2012)MethodsMolBiol.832:315-37.
2.GengF.,WenzelS.,&TanseyW.(2012)AnnuRevBiochem.81:177-201.

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HRP(HorserADIshperoxidase)

Properties:

  • Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget
  • Readilycombineswithhydrogenperoxide(H2O2)toformHRP-H2O2complexwhichcanoxidizevarioushydrogendonors
  • Catalyzestheconversionof:
    • Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts
    • Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL)
    • Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts
  • Highturnoverrateenablesrapidgenerationofastrongsignal
  • 44kDaglycoprotein
  • Extinctioncoefficient:100(403nm)
  • Applications:Westernblot,immunohistochemistry,andELISA

HRPDatasheet

品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究,同时在其他领域也取得了一定成就,包括翻译后修饰,提供甲基化与乙酰基化抗体。其中,细胞应激领域主要包括热休克蛋白(HSP)领域。我们公司不仅在热休克蛋白领域领先全球,而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列,分别涉及抗体、蛋白、酶联免疫吸附试验(ELISA)试剂盒及小分子领域。