当前位置：
首页
>
产品中心 >
Electrophoretic_buffer >
StressMarq/StressXpress® 20S Proteasome Activity Kit/SKT-133-96/96-well
StressMarq/StressXpress® 20S Proteasome Activity Kit/SKT-133-96/96-well
商品编号：
SKT-133-96
市场价：
¥7500.00
美元价：
4500.00
产品分类：
电泳缓冲液
公司分类：
Electrophoretic_buffer
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Overview:
| ProductName | 20SProteasomeActivityKit |
| Description | Fluorometricdetectionofpurified20Sproteasomeactivity |
| SpeciesReactivity | SpeciesIndependent |
| Platform | Microplate |
| SampleTypes | PurifiedProteins |
| DetectionMethod | FluorometricAssay |
| AssayType | ContinuousKineticAssay |
| Utility | Activitykitusedtoscreenpotentialproteasomeinhibitorsandactivators,andtomeasureandquantify20Sproteasomeactivityincontinuouskineticandend-pointassays. |
| IncubationTime | 15-30minutes |
| NumberofSamples | 40samplesinduplicateusingtheplateformat,or100x50µLmicro-cuvettebasedassays |
| OtherResources | KitBooklet,MSDS |
Properties
| StorageTemperature | 4ºC | |||||||||||||||||||||
| ShippingTemperature | BlueIce | |||||||||||||||||||||
| ProductType | ActivityKits | |||||||||||||||||||||
| AssayOverview | The20SProteasomeActivitykitfacilitatestherapid,robustmeasurementofproteasomeactivityinvitro.Thekitutiliseshighpurity,fluorogenicsubstrateSuc-LLVY-AMCtogetherwithsuitablecalibrationstandardsandcontrolsfortheaccurateandsensitiveassessmentofproteasomechymotrypsin-like(β5)activity.Continuouskineticorend-pointassayscanbeperformedinmicro-cuvettesorin96-wellplateformatformulti-sampleanalysis.Containssufficientmaterialsforonefull96-wellplateassayorupto100x50µLmicro-cuvettebasedassaystoberun. | |||||||||||||||||||||
| KitOverview |
| |||||||||||||||||||||
| CiteThisProduct | 20SProteasomeActivityKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-133) |
BIOLOGicalDescription
| AlternativeNames | Chymotrypsin-like(β5)ActivityKit |
| ResearchAreas | Cancer,Apoptosis,CardiovascularSystem,CellSignaling,Neurodegeneration,Neuroscience,Post-translationalModifications |
| ScientificBackground | Proteasomesarenon-lysosomalproteolyticcomplexeslocalisedprimarilyinthecytoplasmandinthenucleusofeukaryoticcells.TheyareresponsIBLefortheubiquitin-mediateddegradationofshorthalf-lifeproteinsandpeptidesthatareinvolvedinessentialcellularprocessesincludingcell-cycleregulation,apoptosisandtranscriptionalregulation,innateimmunityandantigenprocessing,andintheremovalofredundantordamagedproteins.Assuchproteindegradationbytheubiquitin-proteasomepathwayhasamajorregulatoryfunctionforproliferationactivityandsurvivalofbothnormalandmalignantcells,anditsdysfunctionhasbeenimplicatedinawiderangeofotherdiseaseprocessesincludingneurodegenerative,cardiovascularandmetabolicdisorders.The26Sproteasomestructureiscomposedofa20Sproteasomecatalyticcorecomplexandoneortwo19S(PA700)regulatorysubcomplexes.The20Scorecomprisestwocopiesof14subunits(7alphasubunitsand7betasubunits)arrangedinaα7β7β7α7cylindricalarray.Proteolyticactivitiesaredeterminedbytheβ1(caspase-like),β2(trypsin-like)andβ5(chymotrypsin-like)subunits,accesstowhichisguardedbytheα-subunits.The19SregulatoryunitconsistsofsixATPaseandatleasttennon-ATPasesubunitsthatarerequiredforubiquitinatedproteinbinding,deubiquitination,substrateunfoldingandtranslocationtothe20Scatalyticcore.Varyingcatalyticsubunitcomposition(β1,β1i;β2,β2i;β5,β5i)resultsinavarietyofpossiblesubtypesfromfullconstitutiveproteasome(β1,β2,β5)throughmixedpopulationstofullinducible/immunoproteasome(β1i,β2i,β5i).AlternativeregulatorycomplexessuchasthePA200and11Sproteasomeactivatorsconferdifferentsubstratespecificitiesandactivitycomparedtothe19Sregulator. |
| References | 1.Saeki,Y.&Tanaka,K.Methodsinmolecularbiology(Clifton,N.J.)832,315–37(2012). 2.Frankland-Searby,S.&Bhaumik,S.R.Biochimicaetbiophysicaacta1825,64–76(2012). 3.Basler,M.,Kirk,C.J.&Groettrup,M.Currentopinioninimmunology1–7(2012). 4.Löw,P.Generalandcomparativeendocrinology172,39–43(2011). 5.Dennissen,F.J.a,Kholod,N.&VanLeeuwen,F.W.Progressinneurobiology96,190–207(2012). 6.Geng,F.,Wenzel,S.&Tansey,W.P.Annualreviewofbiochemistry81,177–201(2012). |
ProductImages
AMCStandardCurvefor20SProteasomeActivityKit.Representativeplotoffluorescencemeasurements(RFU)ofserialdilutionsofAMCStandardatrecommendedconcentrations.
Continuouskineticenzymeactivityassayshowingthetimecourseofcontrol20SproteasomeactivitywithSuc-LLVY-AMCsubstrateusingtheStressXpress®20SProteasomeActivityKit.20Sproteasome(~2.5nM)wasincubatedwith100µMSuc-LLVY-AMCinproteasomeassaybufferfor30minutesatroomtemperaturealongsideasubstrateonly(blank)controlreaction.Fluorescencemeasurements(RFU)weretakenat30secondintervalsandplottedvs.time.
ProductCitations(0)
Currentlytherearenocitationsforthisproduct.
品牌介绍
StressMarq Biosciences公司的核心技术领域为细胞应激与离子通道以及载体研究，同时在其他领域也取得了一定成就，包括翻译后修饰，提供甲基化与乙酰基化抗体。其中，细胞应激领域主要包括热休克蛋白（HSP）领域。我们公司不仅在热休克蛋白领域领先全球，而且在氧化应激领域也卓有成就。StressMarq的优势在于提供四种独立的产品系列，分别涉及抗体、蛋白、酶联免疫吸附试验（ELISA）试剂盒及小分子领域。
